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Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye
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Biology
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Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

Quantification of dsDNA using the Hitachi F-7000 Fluorescence Spectrophotometer and PicoGreen Dye

DOI:
10.3791/2465-v

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07:44 min

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November 05, 2010

•

,
1Life Sciences Group,Hitachi High Technologies America

Chapters

  • 00:00Title
  • 00:52Instrument Setup
  • 04:50Measurement of Standards and Sample Fluorescence
  • 06:38Microplate Quantification of dsDNA with PicoGreen
  • 07:06Conclusion

Summary

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Demonstration of quantification of dsDNA using Molecular Probes PicoGreen dye and Hitachi F-7000 Fluorescence Spectrophotometer equipped with a microplate reader accessory.

Tags

QuantificationDsDNAHitachi F-7000 Fluorescence SpectrophotometerPicoGreen DyeBiological ApplicationsMolecular Biology AssaysDiagnostic ApplicationsDNA Amplification ProductsDrug PreparationsMicro Plate Reader AccessoryMolecular Probes Quant-it PicoGreen Dye Reagent KitHigh SensitivityHigh Speed MeasurementsFluorescenceLuminescencePhosphorescenceWavelength ScanTime ScanPhotometry3-D Scan MeasurementPicomoles Of FluoresceinSample VolumeMicroplateScan SpeedsDynamic RangeCalibration CurvesReflective Optics

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