Journal
/
Biology
/
使用C2C12成肌细胞的生物能简介实验
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Bioenergetic Profile Experiment using C2C12 Myoblast Cells
Please note that all translations are automatically generated. Click here for the English version.

使用C2C12成肌细胞的生物能简介实验

DOI:
10.3791/2511-v

•

07:20 min

•

December 06, 2010

•

, , , , ,
1Buck Institute for Age Research,Novato, CA, 2Department of Pathology, Center for Free Radical Biology,University of Alabama at Birmingham – UAB, 3Seahorse Bioscience,North Billerica, MA

Chapters

  • 00:05Title
  • 01:04Introduction
  • 02:01Seeding Protocol
  • 03:27Loading Sensor Cartridge and Protocol Commands
  • 04:50Representative Bioenergetic Profiling Results
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

在细胞线粒体功能分析的方法的描述。线粒体产生的个人资料提供线粒体的功能，可以在一次实验中测量的四个参数：基础呼吸速率，ATP挂钩呼吸，质子漏，和储备能力。

Tags

Bioenergetic ProfileExperimentC2C12 Myoblast CellsCellular MetabolismMitochondrial DysfunctionObesityDiabetesAgingCancerCardiovascular FunctionSafety ToxicitySubstrate UptakeOxygenGlucoseFatty AcidsGlutamineEnergy ConversionEnzymatically Controlled Oxidation And Reduction ReactionsATP ProductionHeat ReleaseLactate ProductionCO2 ReleaseExtracellular EnvironmentPhysiological State Of CellsOxygen Consumption Rate (OCR)GlycolysisLactate ProductionProtons ReleaseAcidification Rate (ECAR)Seahorse Cell Culture Plates

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2511?article_type=v

使用人工饲料实验测试蚜虫的尼古丁公差
check_url/2511?article_type=v

Morris水迷宫实验
check_url/2511?article_type=v

真核Polyribosome剖面分析
check_url/2511?article_type=v

斑马鱼胚胎的代谢谱分析
check_url/2511?article_type=v

诱导上皮和分析间质转化
check_url/2511?article_type=v

原发性生肌前体细胞/成肌细胞从基底哺乳动物支系文化准备
check_url/2511?article_type=v

代谢谱的实时分析<em>离体</em>鼠标肠隐窝类器官培养
check_url/2511?article_type=v

肾毒素显微注射斑马鱼到模型急性肾损伤
check_url/2511?article_type=v

胞嘧啶修饰形式使用敏感免疫组化检测
check_url/2511?article_type=v

软件辅助跟踪动态细胞突变中蛋白质浓度的图形用户界面

Read Article