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口腔生物膜的生物化学，结构和转录综合评价分析工具盒介导变形链球菌
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Immunology and Infection
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JoVE Journal Immunology and Infection
An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
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口腔生物膜的生物化学，结构和转录综合评价分析工具盒介导变形链球菌

DOI:
10.3791/2512-v

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11:09 min

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January 25, 2011

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, , ,
1Center for Oral Biology,University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases,Sichuan University, 3Department of General Medicine,Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology,University of Rochester Medical Center

Chapters

  • 00:00Title
  • 02:17Fluorescent Labeling of Exopolysaccharides and Bacterial Cells in Biofilms
  • 04:12Biofilm Image Acquisition and Data Analysis
  • 04:58Biofilm Structural Analysis and Quantification
  • 06:25Transcriptome Analysis of Biofilms
  • 09:51Differential Gene Expression in S. mutans Biofilms Formed in the Presence of Different Dietary Carbohydrates
  • 10:28Conclusion

Summary

Automatic Translation

Automatic Translation

在牙齿表面形成的生物膜是非常复杂和暴露于恒定的先天和外生的环境挑战，调节它们的架构，生理和转录。我们制定了一个工具箱，研究的成分，组织结构和基因表达口腔生物膜，生物膜研究的其他领域可适应。

Tags

Analytical Tool-boxComprehensive Biochemical EvaluationStructural EvaluationTranscriptome EvaluationOral BiofilmsMutans StreptococciMicrobial CellsExtracellular MatrixBiofilm DevelopmentCell ClustersMicrocoloniesExtracellular EnzymesSucrose SubstrateDental BiofilmsExopolysaccharides (EPS)Caries DiseaseGlucosyltransferasesTooth SurfacesEnvironmental ChallengesDiet-host-microbial InteractionsOral CavityMatrix CompositionPhysiology Profile

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