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收集可变浓度等温滴定量热数据集,以确定约束机制
JoVE Journal
Biology
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JoVE Journal Biology
Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
DOI:

10:04 min

April 07, 2011

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Chapters

  • 00:05Title
  • 01:10Introduction
  • 01:28Preparation of Stock Solutions
  • 03:29Prepare the Isothermal Titration Calorimetry Samples
  • 04:48Syringe Set-up
  • 05:33Load the Sample Cell
  • 06:54Load the Injection Syringe and Initiate the Run
  • 08:57Variable Concentration Isotherms and Binding Modeling
  • 09:46Conclusion

Summary

Automatic Translation

ITC是一个功能强大的工具,为研究其主机的一个配体的结合。然而,在复杂的系统的几种模式可能适合同样的数据。这里介绍的方法,提供了一种手段来澄清合适的绑定模型和复杂的系统,提取相应的热力学参数。

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