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Chapters
- 00:05Title
- 00:56Egg Laying and Selecting Larvae
- 02:22Dissection of Larva
- 03:30FIxation and Staining
- 05:20Mounting the Ovaries
- 06:40Results: Somatic and Germ Cells in 3rd Instar Larvae
- 07:20Conclusion
How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development. This video demonstrates how to dissect, stain and mount female gonads from late third instar (LL3) Drosophila larvae.
Tags
DissectionStainingDrosophila Larval OvariesStem CellsDevelopmentEmbryogenesisMaintenanceRepairEnvironmentFruit FlyDrosophila MelanogasterGerm Line Stem Cells (GSCs)Somatic Support CellsNicheGenetic ManipulationInteractionsMechanismsDifferentiationSomatic NichesCellular ComponentsTerminal FilamentCap CellsLarval InstarPrimordial Germ Cells (PGCs)ProliferationFunctional UnitEggs
