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夹层和染色果蝇幼虫卵巢
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JoVE Journal Biology
Dissection and Staining of Drosophila Larval Ovaries
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夹层和染色果蝇幼虫卵巢

DOI:
10.3791/2537-v

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07:40 min

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May 13, 2011

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,
1Department of Biological Regulation,Weizmann Institute of Science

Chapters

  • 00:05Title
  • 00:56Egg Laying and Selecting Larvae
  • 02:22Dissection of Larva
  • 03:30FIxation and Staining
  • 05:20Mounting the Ovaries
  • 06:40Results: Somatic and Germ Cells in 3rd Instar Larvae
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

壁龛和干细胞如何在发展过程中形成的，是一个具有实际意义的重要问题。在<em>果蝇</em>卵巢，生殖系干细胞和体龛的形式在幼虫发育。本视频演示了如何解剖，染色和安装从晚三龄（LL3）女性性腺<em>果蝇</em>幼虫。

Tags

DissectionStainingDrosophila Larval OvariesStem CellsDevelopmentEmbryogenesisMaintenanceRepairEnvironmentFruit FlyDrosophila MelanogasterGerm Line Stem Cells (GSCs)Somatic Support CellsNicheGenetic ManipulationInteractionsMechanismsDifferentiationSomatic NichesCellular ComponentsTerminal FilamentCap CellsLarval InstarPrimordial Germ Cells (PGCs)ProliferationFunctional UnitEggs

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