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Chapters
- 00:05Title
- 01:12Extract Epithelial Stem Cells from Human Skin
- 03:11Culture Primary Epithelial Stem Cells
- 05:19Immortalize Epithelial Stem Cells
- 06:52Skin Epithelial Stem Cell Culture and Differentiation Results
- 08:02Conclusion
En snabb, robust sätt att isolera livskraftiga vuxna epitelceller stamceller från mänsklig hud beskrivs. Metoden utnyttjar enzymatisk nedbrytning av hudens kollagen matrix, följt av plockning av hårsäckarna och isolering av enstaka cellsuspensioner eller fragment vävnad för cellodling.
Tags
IsolationCultureAdult Epithelial Stem CellsHuman SkinHomeostasisAsymmetric DivisionsDaughter CellsStem Cell PhenotypeTransit-amplifying CellsStem Cell NicheRapid ProliferationTerminal DifferentiationEpidermisHair FollicleIntestineIn Vitro Proliferative PotentialLabel-retaining CellsMulti-potent Stem CellsRodent Hair Follicle Stem CellsEpidermisSebaceous GlandsHair FolliclesHuman Hair Follicle Bulge RegionMulti-potentialityBiomedical ResearchDevelopmental BiologyDifferentiationTumorigenesisTherapeutic Utility
