Journal
/
Biology
/
传代人类神经干细胞
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Passaging Human Neural Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

传代人类神经干细胞

DOI:
10.3791/263-v

•

10:16 min

•

August 22, 2007

•

,
1Department of Pathology,University of California, Irvine (UCI)

Chapters

  • 00:02Title
  • 00:07Introduction
  • 01:42Preparing the Coated Flask and Dissociating the Cells
  • 06:14Centrifuging and Resuspending Cells
  • 09:44Counting Cells in Suspension

Summary

Automatic Translation

Automatic Translation

操作能力在体外培养人类神经干/前体细胞（hNSPCs）允许调查作为细胞移植用于治疗目的的实用价值与探索人类的神经系统发育。该协议提出希望增加人类干细胞研究的可重复性的培养和传代hNSPCs的方法。

Tags

PassagingHuman Neural Stem CellsHNSPCsIn VitroCell TransplantsTherapeutic PurposesHuman Neural DevelopmentPathologyCulturingStandardizing TechniqueReproducibilityStem Cell ResearchCadaveric Postnatal Brain CorticesNational Human Neural Stem Cell ResourceAdherent CulturesFibronectin CoatingDMEM:F12 Serum-free MediaEGFFGFPDGFBipolar MorphologyUndifferentiated Neural Stem CellsNestinSox2

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/263?article_type=v

人类胚胎干细胞传代的色调线与胰蛋白酶
check_url/263?article_type=v

人类胚胎干细胞（ES细胞）的繁殖
check_url/263?article_type=v

人类神经干细胞转染与Amaxa Nucleofector
check_url/263?article_type=v

人类神经干细胞计数
check_url/263?article_type=v

免疫组化：人类神经干细胞
check_url/263?article_type=v

人类胚胎干细胞的推导Immunosurgery
check_url/263?article_type=v

小鼠表皮神经嵴干细胞（EPI的非公务员合约雇员）文化
check_url/263?article_type=v

从人类胚胎干细胞的染色质免疫沉淀
check_url/263?article_type=v

文化和维护人类胚胎干细胞
check_url/263?article_type=v

人类胚胎干细胞冷冻和解冻

Read Article