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Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
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Bioengineering
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JoVE Journal Bioengineering
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

DOI:
10.3791/2640-v

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04:24 min

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February 13, 2011

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, , , ,
1Department of Materials Science and Engineering,MIT – Massachusetts Institute of Technology, 2Department of Mechanical Engineering,MIT – Massachusetts Institute of Technology, 3HST Center for Biomedical Engineering and Harvard Stem Cell Institute,Brigham and Women’s Hospital and Harvard Medical School

Chapters

  • 00:05Title
  • 01:26Fabrication of Patterned Substrates
  • 02:26Cell Rolling Experiments in a Flow Chamber
  • 03:24HL60 Trajectories on Asymmetric Receptor Patterns
  • 04:06Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.

Tags

Cell Rolling TrajectoriesAsymmetric Receptor PatternsLabel-free SeparationAnalysis Of CellsLateral DisplacementContinuous-flow SeparationReceptor ExpressionCell PhenotypesAdhesion ModulationCell Rolling AdhesionP-selectin ReceptorsMicrocontact PrintingGold-coated SlidesFlow ChamberHL60 CellsPSGL-1 LigandInclined EdgesPattern EdgesEdge Tracking Length

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