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1Department of Materials Science and Engineering,MIT – Massachusetts Institute of Technology, 2Department of Mechanical Engineering,MIT – Massachusetts Institute of Technology, 3HST Center for Biomedical Engineering and Harvard Stem Cell Institute,Brigham and Women’s Hospital and Harvard Medical School
Chapters
- 00:05Title
- 01:26Fabrication of Patterned Substrates
- 02:26Cell Rolling Experiments in a Flow Chamber
- 03:24HL60 Trajectories on Asymmetric Receptor Patterns
- 04:06Conclusion
Wir beschreiben ein Protokoll zu beobachten und zu analysieren Zellrollen Trajektorien auf asymmetrische Rezeptor-gemusterten Substrate. Die resultierenden Daten sind nützlich für das Engineering von Rezeptor-gemusterten Substrate für Label-freie Zelle Trennung und Analyse.
Tags
Cell Rolling TrajectoriesAsymmetric Receptor PatternsLabel-free SeparationAnalysis Of CellsLateral DisplacementContinuous-flow SeparationReceptor ExpressionCell PhenotypesAdhesion ModulationCell Rolling AdhesionP-selectin ReceptorsMicrocontact PrintingGold-coated SlidesFlow ChamberHL60 CellsPSGL-1 LigandInclined EdgesPattern EdgesEdge Tracking Length
