This content is Free Access.
1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor College of Medicine, 2Texas Children's Cancer Center,Baylor College of Medicine, 3Department of Pediatrics,Baylor College of Medicine, 4Department of Pathology,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor College of Medicine,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor College of Medicine
Chapters
- 00:05Title
- 01:39Preparation of Retinoblastoma Tumorspheres
- 02:54Preparation of Tumor Cells and Animals before Injection
- 04:14Injection of Tumor Cells
- 05:32Monitoring Injected Mice and Harvesting Tumors
- 06:28Retinoblastoma Tumors in Mice
- 07:13Conclusion
A method is described to propagate human retinoblastoma tumors in mice. Tumor cells are directly injected into the eyes of immune deficient mice. Secondary tumors have been successfully established using both cells directly harvested from human tumors and cultured tumorspheres.
