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1Interdepartmental Program in Translational Biology & Molecular Medicine,Baylor College of Medicine, 2Texas Children's Cancer Center,Baylor College of Medicine, 3Department of Pediatrics,Baylor College of Medicine, 4Department of Pathology,The Methodist Hospital Research Institute, 5Department of Ophthalmology,Retinoblastoma Center of Houston, 6Baylor College of Medicine,Center for Cell and Gene Therapy, 7Center for Cell and Gene Therapy,Baylor College of Medicine
Chapters
- 00:05Title
- 01:39Preparation of Retinoblastoma Tumorspheres
- 02:54Preparation of Tumor Cells and Animals before Injection
- 04:14Injection of Tumor Cells
- 05:32Monitoring Injected Mice and Harvesting Tumors
- 06:28Retinoblastoma Tumors in Mice
- 07:13Conclusion
Um método é descrito para propagar tumores retinoblastoma humano em camundongos. Células tumorais são diretamente injetadas nos olhos de ratos imune deficiente. Tumores secundários foram estabelecidas com sucesso usando as duas células diretamente colhidas a partir de tumores humanos e tumorspheres cultivadas.
