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झिल्ली प्रोटीन तह के ऊष्मप्रवैगिकी प्रतिदीप्ति स्पेक्ट्रोस्कोपी द्वारा मापा
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
DOI:

10:09 min

April 28, 2011

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Chapters

  • 00:05Title
  • 00:44Introduction
  • 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
  • 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
  • 04:34Measurement of Fluorescence Spectra
  • 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
  • 08:21Representative Results for the Measurement of Membrane Protein Unfolding
  • 09:22Conclusion

Summary

Automatic Translation

इस वीडियो आलेख विवरण tryptophan प्रतिदीप्ति द्वारा गिब्स झिल्ली प्रोटीन तह मुक्त ऊर्जा प्राप्त करने के लिए प्रयोगात्मक प्रक्रिया.

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