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Bioengineering
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蛍光分光法により測定膜タンパク質フォールディングの熱力学
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Bioengineering
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JoVE Journal
Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
Please note that all translations are automatically generated.
Click here for the English version.
蛍光分光法により測定膜タンパク質フォールディングの熱力学
DOI:
10.3791/2669-v
•
10:09 min
•
April 28, 2011
•
Diana E. Schlamadinger
,
Judy E. Kim
1
Chemistry and Biochemistry
,
University of California San Diego – UCSD
Chapters
00:05
Title
00:44
Introduction
01:24
Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
03:10
Sample Preparation for Initial Fluorescence Unfolding Curve
04:34
Measurement of Fluorescence Spectra
05:45
Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
08:21
Representative Results for the Measurement of Membrane Protein Unfolding
09:22
Conclusion
Summary
Automatic Translation
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トリプトファン蛍光法による膜タンパク質の折り畳みのギブス自由エネルギーを得るためにこのビデオの記事の詳細は、実験手順。
Tags
Thermodynamics
Membrane Protein Folding
Fluorescence Spectroscopy
Gibbs Free Energy
Unfolding
Denaturant
Integral Membrane Protein
E. Coli
Equilibrium Populations
Folded States
Unfolded States
Denaturant Concentration
Lipid Vesicles
Data Analysis Procedure
Temperature
PH
Folding Environments
Lipids
Micelles
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