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蛍光分光法により測定膜タンパク質フォールディングの熱力学
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
DOI:

10:09 min

April 28, 2011

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Chapters

  • 00:05Title
  • 00:44Introduction
  • 01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
  • 03:10Sample Preparation for Initial Fluorescence Unfolding Curve
  • 04:34Measurement of Fluorescence Spectra
  • 05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
  • 08:21Representative Results for the Measurement of Membrane Protein Unfolding
  • 09:22Conclusion

Summary

Automatic Translation

トリプトファン蛍光法による膜タンパク質の折り畳みのギブス自由エネルギーを得るためにこのビデオの記事の詳細は、実験手順。

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