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Chapters
- 00:05Title
- 01:09Agrobacterium Transient Infiltration
- 04:04Assessing Results with a Histochemical GUS Assay
- 04:50Assessing Results with a Fluorometric MUG Assay
- 06:34Cell Death as Measured by GUS Expression
- 07:04Conclusion
Morte cellulare programmata test comunemente usati nei sistemi di mammiferi, come laddering DNA o saggi TUNEL, sono spesso difficili da riprodurre nelle piante. In combinazione con un sistema di giornalista GUS, proponiamo un rapido test basato impianti transitori per analizzare le proprietà morte potenziale di specifici geni.
Tags
A β-glucuronidase (GUS)Transient Plant Expression SystemReporter GenePositive Regulators Of Cell DeathN. Benthamiana LeavesCo-infiltration35S Driven Expression CassetteGene AnalysisGUS Vector PCAMBIA 2301Agrobacterium Strain LBA4404Live CellsMarker GeneAnti-apoptotic GenesPro- And Anti-death PlayersBcl-2 Associated AthanoGene (BAG) FamilyMammalian Inducers Of Cell DeathBAXPost-translational Modification/activation Of ProteinsRapid And Sensitive Plant Based Method
