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使用荧光免疫化学检测爱德华ictaluri（一毛虫）样品
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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
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使用荧光免疫化学检测爱德华ictaluri（一毛虫）样品

DOI:
10.3791/2687-v

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05:23 min

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May 10, 2011

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,
1Department of Basic Sciences,Mississippi State University

Chapters

  • 00:05Title
  • 01:15Bacterial Challenge and Tissue Sampling
  • 02:29Dewaxing the Sections and Immunohistochemistry
  • 04:26Representative Fluorescent Images
  • 04:48Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一个程序，允许标签<em爱德华ictaluri原位</em在斑点叉尾鮰的组织切片<em>斑点叉尾毛虫</em>作为一个小学，和荧光FITC标记抗体作为辅助用单克隆抗体间接免疫组化Ed9。这允许使用荧光显微镜观察细菌的检测。

Tags

Fluorescent Immuno-ChemistryDetectionEdwardsiella IctaluriChannel CatfishIctalurus PunctatusMethod DevelopmentIn Situ VisualizationBacteriaHistological SectionsBacterial LocalizationPlate CountsRadiometric LabeledBioluminescent BacteriaGross Organ LevelInfection CycleVirulence FactorsInteraction With HostSalmonella TyphiTaxonomic FamilyTechnique DescriptionIndirect Immuno-histochemistryMonoclonal Ed9 AntibodyBlocking SerumParaffin Embedded Histological SectionsPrimary AntibodySecondary AntibodiesFitC Labeled Secondary Antibodies

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