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偏光易位荧光蛋白爪蟾外胚层响应Wnt信号
JoVE Journal
Biology
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JoVE Journal Biology
Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling
DOI:

06:55 min

May 26, 2011

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Chapters

  • 00:05Title
  • 01:00Introduction
  • 01:27In vitro Fertilization of Xenopus Eggs
  • 02:34RNA Microinjection
  • 03:46Prepare Ectodermal Explants for Imaging
  • 04:46Alternative Approach: Prepare Cryosectioned Embryos for Imaging
  • 05:57Representative Fluorescent Imaging Results
  • 06:29Conclusion

Summary

Automatic Translation

非洲爪蟾胚胎外胚层已经成为一个有吸引力的模型研究细胞极性。描述一个试验,荧光蛋白的亚细胞分布在外胚层细胞进行评估。该协议将有助于地址问题涉及到空间的信号控制。

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