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MG53介导的细胞膜使用维修的可视化在体内在体外系统
JoVE Journal
Biology
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JoVE Journal Biology
Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
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MG53介导的细胞膜使用维修的可视化在体内在体外系统

DOI:
10.3791/2717-v

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10:19 min

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June 30, 2011

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, , , , ,
1Department of Physiology and Biophysics,Robert Wood Johnson Medical School

Chapters

  • 00:05Title
  • 01:25Treadmill Running
  • 03:12Membrane Repair Capacity of Isolated Muscle Fibers Following UV-Laser Damage
  • 07:25Live Cell Confocal Imaging to Monitor the Dynamic Process of Cell Membrane Repair
  • 08:41Reduced Running Capacity and Compromised Membrane Repair Capacity
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

这里描述的是MG53介导的细胞在整个动物的膜修复，并在细胞水平上的动态过程可视化的协议。这些方法可以适用于调查的质膜再加盖印章和再生医学细胞生物学。

Tags

MG53Cell Membrane RepairIn Vivo SystemIn Vitro SystemAcute InjuryCellular PhysiologyDefective Membrane RepairDegenerative Human DiseasesMolecular UnderstandingRegenerative MedicineExperimental ProtocolsMuscle InjuryTreadmill Exercise ProtocolsMouse ModelsEx Vivo Membrane Repair CapacityDye EntryIsolated Muscle FibersVesicle TraffickingLive Cell Confocal Microscopy

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