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Bioengineering
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一个简单的悬滴细胞生成三维球体文化协议
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
Please note that all translations are automatically generated.
Click here for the English version.
一个简单的悬滴细胞生成三维球体文化协议
DOI:
10.3791/2720-v
•
07:24 min
•
May 06, 2011
•
Ramsey Foty
1
Department of Surgery
,
UMDNJ-Robert Wood Johnson Medical School
Chapters
00:05
Title
01:14
Preparing a Single Cell Suspension
02:57
Hanging Drop Formation
04:38
Representative Results of Hanging Drop Cultures, Compaction of Cellular Aggregates and Spheroid Bodies
07:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个简单快速的方法,生成三维组织类似的球体和其潜在的应用,量化,在细胞间的相互作用的差异。
Tags
Hanging Drop Cell Culture
3D Spheroids
Cell-cell Cohesion
Cell-substratum Adhesion
Monolayer Culture
Rigid Substrates
Intimate Connections
Extracellular Matrix Components
Chemical Milieu
Physical Forces
Cellular Morphology
Signaling
Collagen Gels
Biomaterial Scaffolds
Direct Cell-cell Adhesion Architecture
Culture Systems
ECM Products
Physiological Conditions
Biological Agent
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