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Biology
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确定基因表达概况
C。线虫
使用微阵列和实时PCR
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Determining Genetic Expression Profiles in
C. elegans
Using Microarray and Real-time PCR
Please note that all translations are automatically generated.
Click here for the English version.
确定基因表达概况
C。线虫
使用微阵列和实时PCR
DOI:
10.3791/2777-v
•
10:27 min
•
July 30, 2011
•
Kassandra L. Guthmueller
,
Maggie L. Yoder
,
Andrea M. Holgado
1
Department of Biological Sciences
,
Southwestern Oklahoma State University
Chapters
00:05
Title
01:12
RNA Preparation
03:32
Microarray Hybridization
05:32
Microarray Analysis
08:44
Finding Gene Expression Changes in the vsm-1 Synaptogenesis Mutant
10:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
进行微阵列分析,以确定基因的表达谱<em> C。线虫</em>,并实时PCR是用来验证和量化的微阵列数据。
Tags
Genetic Expression Profiles
C. Elegans
Microarray
Real-time PCR
Synapses
Presynaptic Active Zone
Postsynaptic Terminal
Neurotransmitters
Receptors
VSM-1
Exocytosis Regulation
Synaptic Connectivity
Synaptogenesis
Double-labeled Microarray Analysis
Gene Expression Profiles
RNA Isolation
CDNA Reverse Transcription
DNA Microarrays
Fluorescent Probe Hybridization
Major Sperm Protein Family (MSP)
Nematode Oocyte Maturation
Ovulation
Presynaptic Bouton Number And Size
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