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Chapters
- 00:05Title
- 01:18Production of Lentivirus
- 04:03Titering the Lentivirus
- 04:57Transduction of Human Target Cells with Murine Retrovirus Receptor Slc7a1
- 05:50Polymer Complex Titration to Determine Toxicity
- 10:15Ecotropic Transduction with Polymer Complexation
- 11:08Results of the Procedure
- 13:32Conclusion
Tags
TransductionHuman CellsPolymer-complexedEcotropic LentivirusEnhanced BiosafetyStem Cell BiologyTumor Cell BiologyViral TransductionOncogenesBiosafety Level HandlingPantropic LentivirusesVSV-G PseudotypeGene FunctionProduction And CentrifugationInduced Pluripotent Stem Cells (iPSC)Oncogene Encoding VirusesGenetic ChangesPluripotencyCancer-related VirusesBiosafety ConcernsCellular ReprogrammingReduced CostConvenient HandlingHigh Titer
