/

/

在富含GC KISS1受体序列与人类生殖系统紊乱中确定的诱变和基因突变分析

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders

Please note that all translations are automatically generated. Click here for the English version.

在富含GC KISS1受体序列与人类生殖系统紊乱中确定的诱变和基因突变分析

DOI:

•

12:49 min

•

September 04, 2011

•

Luciana Madeira da Silva, Lauren Vandepas, Suzy D.C. Bianco²

¹Division of Endocrinology and Metabolism, Department of Medicine,University of Miami Miller School of Medicine, ²Department of Molecular and Cellular Pharmacology,University of Miami Miller School of Medicine

Chapters

00:05Title
02:03Site-directed Mutagenesis of Highly GC-rich KISSR Gene Sequence
03:48Cell Treatment and Lysis
06:24Immunoprecipitation of MycKISS1R
07:16Western Blot Detection of MycKISS1R Immunocomplexes
08:45Imaging and Quantification of MycKISS1R
10:10Analysis of KISS1R
11:46Conclusion

Summary

Automatic Translation

kisspeptin受体（KISS1R）的突变与患者的生殖系统紊乱。在这里，我们描述如何引入利益的突变的KISS1R富含GC的序列以及使用KISS1R结构特点免疫沉淀和免疫印迹的受体的降解途径

Tags

Mutagenesis Genetic Mutations GC-rich KISS1 Receptor Reproductive Disorders Kisspeptin Receptor Inactivating Mutations Hypogonadotropic Hypogonadism Precocious Puberty Functional Studies Regulation Of Reproduction KISS1R Gene PCR Amplification PCR Enhancer Solution DMSO Expression Vector

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

原代培养及质粒电穿孔尔蒂小鼠器官。

异源表达在哺乳动物细胞离子通道的突变和功能分析

作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

在毛发再生的上皮 - 间充质信号的快速遗传分析

另类文化的人多能干细胞的生产，维护和遗传分析

高分辨率成像定时，并在活体人脐静脉内皮细胞微管动力学自动分析

遗传和生物化学方法<em>在体内</em>和<em>体外</em>蛋白齐聚评估：兰尼碱受体案例研究

与VirusMapper超分辨率显微镜开源单粒子分析

用 RNA原位杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化

使用两步PCR和下一代16S rRNA基因测序进行微生物群分析

Read Article