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在细胞培养中缺氧诱导和测试
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Induction and Testing of Hypoxia in Cell Culture
Please note that all translations are automatically generated.
Click here for the English version.
在细胞培养中缺氧诱导和测试
DOI:
10.3791/2899-v
•
07:01 min
•
August 12, 2011
•
Danli Wu
,
Patricia Yotnda
1
Center for Cell and Gene Therapy
,
Baylor College of Medicine
Chapters
00:05
Title
01:10
Inducing Hypoxia Using CoCl2 Solution and a Modular Incubator Chamber
03:04
Evaluating Hypoxia
04:57
Measuring Luciferase Activity
05:35
Hypoxia Detection Results
06:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出简单的方法,诱导缺氧细胞培养和简单的测试,以评估文化的缺氧状态。
Tags
Induction
Testing
Hypoxia
Cell Culture
Oxygen Reduction
Oxygen Tension
Supply
Blood Vessels
Anemia
Consumption
Proliferation Rate
Physiologic
Pathologic
Solid Cancers
Rheumatoid Arthritis
Atherosclerosis
Adaptation Ability
Hypoxia Inducible Factor Alpha (HIF)
Stabilization
Angiogenesis
Oxygen Transport
Protein Detection
Screening
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