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蛋白膜覆盖含量:一个协议测试可溶性和不溶性蛋白质之间的相互作用在体外</em
JoVE Journal
Biology
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JoVE Journal Biology
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
DOI:

08:38 min

August 14, 2011

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Chapters

  • 00:05Title
  • 01:10Expression and Extraction of the Proteins
  • 03:22Immobilization of ProIM and ProIMnc on the Membrane
  • 03:54Re-folding of Membrane-bound Proteins
  • 04:49Probing the ProIM by ProSOL
  • 05:33Visualizing Protein-protein Interaction by Immunoblotting
  • 07:06Representative Results
  • 08:20Conclusion

Summary

Automatic Translation

测试是必不可少的蛋白质 - 蛋白质相互作用蛋白质功能的夹层。在这里,我们引入<em>在体外</em>蛋白质 - 蛋白质结合检测探头与可溶性蛋白的膜固定蛋白质。这个实验提供了一个可靠的方法来测试不溶性蛋白质在溶液中的蛋白质之间的相互作用。

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