Journal
/
Medicine
/
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

DOI:
10.3791/3119-v

•

07:51 min

•

September 22, 2011

•

, , ,
1Julius L. Chambers Biomedical/Biotechnology Research Institute,North Carolina Central University, Durham, 2Department of Biology,North Carolina Central University, Durham, 3Department of Physiology & Pharmacology and Hypertension & Vascular Research Center,Wake Forest University School of Medicine

Chapters

  • 00:05Title
  • 01:43Isolation of Rat Mesenteric Small Artery
  • 02:45Mounting of Vessel
  • 04:43Normalization
  • 05:31Measurement of Responses
  • 06:24Contraction and Relaxation in Mesenteric Arteries
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.

Tags

Mesenteric ArteryContractionRelaxationAutomated Wire MyographyProximal Resistance VesselsBlood FlowRat Mesenteric ArteryMyography TechniqueIsometric ConditionsForce ProductionSensitivityAgonistsStretchCompliance Of Mounting WiresStainless Steel WiresTungsten WiresOxidationRecorded ResponsesVascular Smooth Muscle Cell ReceptorsPhenylephrineExtracellular Calcium (Ca2+e)

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3119?article_type=v

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas
check_url/3119?article_type=v

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
check_url/3119?article_type=v

Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine
check_url/3119?article_type=v

Murine Model of Femoral Artery Wire Injury with Implantation of a Perivascular Drug Delivery Patch
check_url/3119?article_type=v

A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury
check_url/3119?article_type=v

Induction of Accelerated Atherosclerosis in Mice: The “Wire-Injury” Model
check_url/3119?article_type=v

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
check_url/3119?article_type=v

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
check_url/3119?article_type=v

Brachial Artery Catheterization in Swine
check_url/3119?article_type=v

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

Read Article