A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:43Preparation of Chemotactic Competent Cells of Dictyostelium discoideum
- 02:57Imaging Chemotaxing Cells in a Visible and Manipulatable Chemoattractant Gradient
- 04:16Immobile Nonpolarized Cell Systems Facilitate Imaging Signaling Events Involved in cAMP Gradient Sensing
- 05:05Representative Images of cAMP Gradient-induced PIP3 Expression
- 07:58Conclusion
Here, we describe detailed live cell imaging methods for investigating chemotaxis. We present fluorescence microscopic methods to monitor spatiotemporal dynamics of signaling events in migrating cells. Measurement of signaling events permits us to further understand how a GPCR-signaling network achieves gradient sensing of chemoattractants and controls directional migration of eukaryotic cells.
