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在体内
肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
In vivo
Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
Please note that all translations are automatically generated.
Click here for the English version.
在体内
肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注
DOI:
10.3791/3138-v
•
12:35 min
•
November 10, 2011
•
Sandhya Gopalakrishnan
,
Edward N. Harris
1
Department of Biochemistry
,
University of Nebraska, Lincoln
Chapters
00:05
Title
02:37
Purification of Hepatocytes and SECs from Rat Liver
04:18
Internalization of 125I-SA-b-Hep or Other Suitable Labeled Ligand
06:55
Hepatocyte and Sinusoidal Endothelial Cell (SEC) Purification
11:08
Distribution of Biotin-Labeled Heparin
12:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
从动物获得的无细胞系存在的主要细胞,肝窦内皮细胞(秒)的研究必须执行。这种方法依赖于肝的消化和差速离心SEC随后的培养和实验净化。
Tags
In Vivo Liver Endocytosis
Purification Of Liver Cells
Liver Perfusion
Hepatocytes
Kupffer Cells
Stellate Cells
Sinusoidal Endothelial Cells
Fenestrae
Cytoplasmic Holes
Space Of Disse
Scavenger Receptors
SR-A
Stabilin-1
Stabilin-2
Endocytosis
Phagocytosis
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