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Neuroscience
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使用调频染料培养的小脑颗粒神经突触小泡池补充的定量分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
Please note that all translations are automatically generated.
Click here for the English version.
使用调频染料培养的小脑颗粒神经突触小泡池补充的定量分析
DOI:
10.3791/3143-v
•
09:02 min
•
November 11, 2011
•
Giselle Cheung
,
Michael A. Cousin
1
Membrane Biology Group, Centre for integrative Physiology
,
University of Edinburgh
Chapters
00:05
Title
02:02
Experimental Setup
02:36
Sample Preparation
04:32
S1 Phase
06:12
Data Analysis
Summary
Automatic Translation
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Automatic Translation
描述一个生活的荧光成像技术,量化的补给和动员特定的突触小泡(SV)池,在中央的神经末梢。两轮的SV回收进行监控,在相同的神经终端提供内部控制。
Tags
Quantitative Analysis
Synaptic Vesicle Pool Replenishment
Cultured Cerebellar Granule Neurons
FM Dyes
Neurotransmitter Release
Endocytosis
Recycling Pool
Readily Releasable Pool
Reserve Pool
Assay
SV Traffic
Clathrin-dependent Endocytosis
Activity-dependent Bulk Endocytosis
Mobilisation Mechanisms
SV Turnover
Central Nerve Terminals
Hydrophobic Hydrocarbon Tail
Hydrophilic Head Group
Fluorescent Probes
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