A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:06Introduction
- 00:51Cardiac Puncture and Dissection of Thymus
- 02:52Isolation of Antigen Presenting Cells (APCs) from Thymus
- 06:24Activation of OVA-specific T cells by Thymic APCs
- 12:55Adoptive Transfer of OVA-specific T cells
- 19:27Challenging with OVA in the Ear
- 21:02Measurment of Inflammation in the Ear
- 21:27Conclusion
Type de l'hypersensibilité retardée (DTH) est une réaction inflammatoire médiée par CCR7-effectrices T mémoire (TEM) des lymphocytes. Ici nous démontrons comment activer les cellules spécifiques de l'antigène TEM, induisent SRD adoptifs chez le rat Lewis et de surveiller la réponse inflammatoire.
Tags
InductionMonitoringAdoptive Delayed-type HypersensitivityRatsDTHInflammatory ReactionCCR7-Effector Memory T LymphocytesAntigenImmune SystemImmunosuppressantsCutaneous DTHAdoptive TransferActive ImmunizationIntradermal ChallengeOvalbuminTuberculinTetanus ToxoidKeyhole Limpet HemocyaninAutoantigenMyelin Basic Protein (MBP)Experimental Autoimmune Encephalomyelitis (EAE)Lewis RatsStimulationOvalbumin-specific T CellsIntraperitoneally InjectionEquilibrate In VivoPinnaSaline3-72 Hours Later
