Journal
/
Medicine
/
的方向转变，并在电脉冲电场方向的应用，提高质粒的基因转移在体外</em
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
Please note that all translations are automatically generated. Click here for the English version.

的方向转变，并在电脉冲电场方向的应用，提高质粒的基因转移在体外</em

DOI:
10.3791/3309-v

•

04:46 min

•

September 12, 2011

•

, , , ,
1Department of Fundamentals of Electrical Engineering, Mathematics and Physics,University of Ljubljana, 2Department of Biomedical Engineering,University of Ljubljana

Chapters

  • 00:01Title
  • 00:04Introduction
  • 00:58Cell Culture Preparation for the Experiment
  • 01:42Hardware Equipment
  • 02:09Gene Electrotransfer Protocol
  • 03:31Image Acquisition and Determination of Gene Electrotransfer Efficacy

Summary

Automatic Translation

Automatic Translation

基因转染，电是提高约两​​次脉冲的应用过程中改变电场方向时，细胞活力不受影响。基因转染的增加是由主管DNA进入细胞的膜面积增加。

Tags

Electric FieldGene TransferPlasmid GeneIn VitroGene ElectrotransferCell MembraneDNA EntryViral VectorsElectric Pulse ProtocolsMembrane AreaGene TransfectionGreen Fluorescent Protein

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在免疫缺陷小鼠的建立和传播人类视网膜母细胞瘤肿瘤

缺血性心力衰竭在临床前模型的转基因

在小鼠永久性大脑中动脉结扎术中的应用

在癌症的生产和检测活性氧（ROS）的

在动物模型揭示神经，血液，创伤后应激障碍和行为相关因素的生物标志物

新洲形成的助手依赖型腺病毒载体介导的基因转移对糖尿病小鼠肝

从金属支架表面使用的腺病毒载体栓通过水解的交联剂血管基因转移

一种新型的<em>在体内</em>基因转移技术和<em>在体外</em>细胞分析为肌肉骨骼疾病骨质流失的研究

从人类肿瘤异种移植的小鼠细胞的消耗显着改善靶细胞下游分析

以显微镜为基础的方法评估上皮细胞迁移过程中的体外创面愈合

Read Article