Journal
/
Immunology and Infection
/
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

DOI:
10.3791/3321-v

•

06:38 min

•

November 08, 2011

•

, , , ,
1Stony Brook Children’s Hospital,State University of New York at Stony Brook, 2Department of Pediatrics,State University of New York at Stony Brook, 3Department of Molecular Genetics,State University of New York at Stony Brook, 4Department of Microbiology,State University of New York at Stony Brook

Chapters

  • 01:35Isolation of Peripheral Blood Mononuclear Cells (PBMC)
  • 02:53Infection with Epstein-Barr Virus (EBV)
  • 03:47Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
  • 04:46Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
  • 05:58Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for generating transformed B cell lines using Epstein-Barr virus. We also illustrate a novel assay that can identify B cells destined to undergo transformation as early as three days after infection.

Tags

Epstein-Barr VirusGrowth-transformed Lymphoblastoid Cell LinesEBV InfectionB Cell ProliferationImmortalizationEBV LatencyVirus-driven B Cell ProliferationTumorigenesisImmunologic AssaysHuman Monoclonal AntibodiesMitogensPhytohemagglutininLipopolysaccharidePokeweed MitogenCyclosporin AT Cell-mediated KillingHigh Titer EBVImmunosuppressive AgentPeripheral Blood Mononuclear Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3321?article_type=v

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
check_url/3321?article_type=v

Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
check_url/3321?article_type=v

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
check_url/3321?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/3321?article_type=v

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
check_url/3321?article_type=v

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
check_url/3321?article_type=v

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
check_url/3321?article_type=v

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
check_url/3321?article_type=v

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
check_url/3321?article_type=v

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Read Article