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Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals

DOI:
10.3791/3336-v

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08:30 min

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October 31, 2011

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, , , , , ,
1Laboratoire d’Imagerie et de Modélisation en Neurobiologie et Cancérologie,UMR8165 Université Paris Sud 11, Paris Diderot 7 – CNRS

Chapters

  • 00:05Title
  • 01:47Preparing the Animal for Imaging
  • 03:12Creating a Cranial Window
  • 05:21Optical Imaging for Olfactory Activity Mapping
  • 06:14Representative IOS and FAS Images of Odor-Evoked Activated Zones
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents the protocols of intrinsic optical signals and flavoproteins autofluorescence signals imaging to map odor-evoked activities at the surface of the olfactory bulb in mice.

Tags

ImagingOdor-Evoked ActivitiesMouse Olfactory BulbOptical ReflectanceAutofluorescence SignalsSensory StimulationDistributed Populations Of NeuronsFunctional ModulesCoding Of The StimulusFunctional Optical Imaging TechniquesSensory CorticesSpatial ResolutionEndogenous Optical SignalsMolecular MechanismsNeuroenergeticsContrastSpatial MapsRodent BrainIntrinsic Optical Signals (IOS)Red Light ReflectanceBlood Oxygenation LevelBlood VolumePhoton ScatteringIn Vivo IOS ImagingWhisker BarrelsOrientation ColumnsOlfactory Bulb (OB)OdorantsLarry Katz’s GroupFlavoprotein Autofluorescence Signals (FAS)Redox StateMitochondrial Metabolic Intermediates

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