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Brug af omvendt genetik til at manipulere NSS Gene af Rift Valley Fever virus MP-12 Strain to Improve Vaccine sikkerhed og effekt
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Brug af omvendt genetik til at manipulere NSS Gene af Rift Valley Fever virus MP-12 Strain to Improve Vaccine sikkerhed og effekt

DOI:
10.3791/3400-v

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09:13 min

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November 01, 2011

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, , ,
1Department of Pathology,University of Texas Medical Branch

Chapters

  • 00:05Title
  • 01:57Preparation of Recombinant MP-12 Encoding NSs Mutations
  • 03:38Amplification of P0 Virus
  • 04:16Titration of Recombinant MP-12 by Plaque Assay
  • 06:01Screening of NSs Mutants Lacking the Type-I IFN Suppression Function
  • 07:03Reverse Genetics Approach to the C13type MP-12 Virus
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

Det omvendte genetik system for Rift Valley fever virus MP-12 vaccinestamme er et nyttigt værktøj til at skabe ekstra MP-12 mutanter med øget dæmpning og immunogenicitet. Vi beskriver protokollen til at generere og karakterisere NSS mutant stammer.

Tags

Reverse GeneticsNSs GeneRift Valley Fever VirusMP-12 StrainVaccine SafetyVaccine EfficacyHemorrhagic FeverNeurological DisordersBlindnessAbortionFetal MalformationHHS/USDA Overlap Select AgentRisk Group 3 PathogenPhlebovirusBunyaviridae FamilyVirulentRVFV MP-12 Vaccine StrainWild-type RVFV StrainsZH548ZH501M-segmentL-segmentS-segment RNA3Functional Virulence Factor NSsRMP12-C13typeIn-frame Deletion Of NSs ORFHost Transcription Shut-offInterferon (IFN)-beta MRNA78Double-stranded RNA-dependent Protein Kinase (PKR)Post-translational Level Degradation Of PKR

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