A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:06Introduction
- 00:48Preparing the Cassettes
- 01:56Pouring the Resolving Gel
- 04:01Pouring the Stacking Gel
- 06:00Storing the Gel
- 06:32Preparing the Samples
- 07:35Running the Gel
- 09:13Staining the Gel
- 10:17Cleaning the Cassettes
- 10:35Conclusion
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
