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Biology
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血管钙的可视化
2 +
信号旁分泌源性活性氧引发
JoVE Journal
Biology
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JoVE Journal
Biology
Visualization of Vascular Ca
2+
Signaling Triggered by Paracrine Derived ROS
Please note that all translations are automatically generated.
Click here for the English version.
血管钙的可视化
2 +
信号旁分泌源性活性氧引发
DOI:
10.3791/3511-v
•
07:33 min
•
December 21, 2011
•
Karthik Mallilankaraman
,
Rajesh Kumar Gandhirajan
,
Brian J. Hawkins
,
Muniswamy Madesh
1
Department of Biochemistry
,
Temple University
,
2
Department of Anesthesiology and Pain Medicine
,
University of Washington
Chapters
00:05
Title
01:18
Oxidation-sensitive Dyes: Visualization of ROS in J774 Cells
02:24
Fluorescent Sensor HyPer: Visualization of ROS in Stably Transfected MPMVECs
04:04
Co-Culture Model for Paracrine-Derived ROS
05:13
ROS Dynamics
06:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种有效的方法获得可视化的旁分泌衍生活性氧诱导内皮细胞Ca2 +信号的见解的描述。这种方法测量旁分泌衍生活性氧引发共培养模型在血管内皮细胞内Ca2 +动员的优势。
Tags
Vascular Ca2+ Signaling
Paracrine
ROS (reactive Oxygen Species)
Oxidative Stress
Ischemia/reperfusion Damage
Sepsis
Signal Transduction
Cell Proliferation
Cell Death
Inflammatory Cells
Cytokines
Tumor Necrosis Factor-alpha (TNF-alpha)
IL-1
Endothelial Cells (EC)
Endothelial Dysfunction
Macrophages
NADPH Oxidases
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