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Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

DOI:

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09:52 min

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November 25, 2011

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Christine M. O’Brien, Kyle Rood, Shramik Sengupta, Sagar K. Gupta, Thiago DeSouza, Aaron Cook, John A. Viator

¹Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology,University of Missouri

Chapters

00:05Title
01:20Blood Sample Preparation
02:58Flow Chamber Construction
04:32Photoacoustic Flowmetry Set-up
06:42Immunocytochemistry
08:39Results: Melanoma Isolation
09:30Conclusion

Summary

Automatic Translation

We have developed a flow cytometer using laser induced ultrasound to detect circulating melanoma cells as an early indicator of metastatic disease.

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