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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Bioengineering
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JoVE Journal Bioengineering
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

DOI:
10.3791/54102-v

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07:37 min

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June 26, 2016

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, , , , ,
1Department of Experimental, Diagnostic and Specialty Medicine – DIMES,University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine,University of Ferrara, 3Department of Life Sciences and Biotechnology,University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA),University of Ferrara

Chapters

  • 00:05Title
  • 01:21Droplet Generation and PCR
  • 03:50Droplet Reading
  • 05:29Absolute Quantification of microRNA in Plasma Samples
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

Tags

Keywords: Circulating MicroRNADroplet Digital PCRDNA-binding Dye ChemistryBiomarkerCancer DiagnosisPlasmaSerumReverse TranscriptionDdPCR MixCDNA TemplateDroplet Generation

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