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Journal
/
Medicine
/
在干细胞移植
在体外
模拟缺血/再灌注模型
JoVE Journal
Medicine
Author Produced
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JoVE Journal
Medicine
Stem Cell Transplantation in an
in vitro
Simulated Ischemia/Reperfusion Model
Please note that all translations are automatically generated.
Click here for the English version.
在干细胞移植
在体外
模拟缺血/再灌注模型
DOI:
10.3791/3575-v
•
09:15 min
•
November 05, 2011
•
Attila Cselenyák
,
Zsolt Benko
,
Mónika Szepes
,
Levente Kiss
,
Zsombor Lacza
1
Institute of Human Physiology and Clinical Experimental Research
,
Semmelweis University
Chapters
00:05
Title
02:20
Preparing H9c2 Cardiomyoblast Cells
03:44
Simulated Ischemia/Reoxygenation
05:25
Preparing Rescue Mesenchymal Stem Cells
06:21
Live/Dead Staining of Co-cultivated Cells
07:23
Analysis with Confocal Microscopy
07:47
Analysis with Flow Cytometry
08:36
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们演示了如何建立一个<em>在体外</em>缺血/再灌注模型,以及如何评价干细胞治疗缺血心肌细胞的影响。
Tags
Stem Cell Transplantation
In Vitro
Simulated Ischemia/reperfusion Model
Clinical Practice
Robust Protocols
New Sources
Implantable Cells
Cell Therapies
Treatment Process Mechanisms
Experimental Protocol
Ischemia/reperfusion Injury
Stem Cell Transplantation Efficacy
H9c2 Cardiomyoblast Cells
Cell Culture Plates
Glucose Free Medium
Oxygen Levels
Reperfusion Simulation
Damaged Cells Treatment
Labeled Human Bone Marrow Derived Mesenchymal Stem Cells
Co-cultivation
Live And Dead Cells Differentiation
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