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Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
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Chapters
- 00:05Title
- 01:00Generation of a Stable Cell Line
- 02:50Silanize the Glass-bottom Dish
- 04:03Preparation of 3D-collagen Gel With Tracer Particles
- 05:35Time-lapse Image Acquisition
- 06:29Inhibitor-addition Experiments
- 07:11FRAP Procedure and Analysis
- 08:15Following Proteins in 3D Cell Migration
- 10:06Conclusion
Cellular processes such as cell migration have traditionally been studied on two-dimensional, stiff plastic surfaces. This report describes a technique for directly visualizing protein localization and analyzing protein dynamics in cells migrating in a more physiologically relevant, three-dimensional matrix.
Tags
Live-cell ImagingMigrating CellsFluorescently-tagged ProteinsThree-dimensional MatrixCell MigrationBiological ProcessesWound HealingCancer MetastasisProtein LocalizationProtein DynamicsExtracellular MatrixOptically Clear MatrixProtein FunctionsGenetically Encoded Fluorescent TagsSubcellular AccumulationCytoskeletal Components
