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Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
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Chapters
- 00:05Title
- 01:25Isolating Adipose-derived Stem Cells
- 02:40Preparing Chitosan Microspheres
- 04:00Quantify Free Amino Groups in CSM
- 05:20Loading ASC in CSM
- 06:11Quantify ASC Loading and Cell Viability in CSM
- 07:17Characterizing ASC-CSM-embedded Collagen Gel
- 08:04ASC-CSM-collagen Gel
- 08:55Conclusion
A major hurdle in current stem cell therapies is determining the most effective method to deliver these cells to host tissues. Here, we describe a chitosan-based delivery method that is efficient and simple in approach, while allowing adipose-derived stem cells to maintain their multipotency.
Tags
Collagen HydrogelStem Cell-loaded Chitosan MicrospheresRegenerative MedicineImplantation SiteMechanical PropertiesVascularizationExtracellular MatrixBiocompatible MaterialsChemical CompositionMechanical StrengthPermeabilityOxygen And NutrientsChitosanCationic PolysaccharideChemical ModificationIn Vivo MacromoleculesGlycosaminoglycan PortionSubstrate For Cell AdhesionMigration And ProliferationAdipose-derived Stem Cells (ASC)Three-dimensional Scaffold
