A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:46Production of Culture
- 02:53Culture Preparation and Mixing (Mating)
- 04:27Isolation of Trans-integrants
- 05:55Results of Integrase Mediated Cassette Exchange (IMCE)
- 08:01Conclusion
Tags
Site-specificBacterial Chromosome EngineeringΦC31 IntegraseCassette ExchangeAttBAttPRecombinationLanding PadSpectinomycin-resistance GeneAadAE. Coli ß-glucuronidase GeneUidASinorhizobium MelilotiOchrobactrum AnthropiAgrobacterium TumefaciensAmpC LocusTetA LocusMobilizable Donor VectorsStuffer Red Fluorescent Protein (rfp) GeneAntibiotic Resistance GeneGentamicin Resistant Plasmid PJH110SphIPstI
