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Journal
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Immunology and Infection
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小鼠腘窝淋巴结的活体成像
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Intravital Imaging of the Mouse Popliteal Lymph Node
Please note that all translations are automatically generated.
Click here for the English version.
小鼠腘窝淋巴结的活体成像
DOI:
10.3791/3720-v
•
08:41 min
•
February 08, 2012
•
H. L. Rachel Liou
,
Jay T. Myers
,
Deborah S. Barkauskas
,
Alex Y. Huang
1
Department of Pediatrics
,
Case Western Reserve University
,
2
Department of Pediatrics, Pathology and Biomedical Engineering
,
Case Western Reserve University
Chapters
00:05
Title
01:13
Mouse Holder Assembly
01:58
Mouse Preparation
03:27
Surgery
04:55
2-Photon Imaging Acquisition
05:55
Migration Analysis of GFP+ Lymphocytes in the Popliteal Lymph Node
08:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
2 - 光子显微镜的最新进展已启用实时<em>在</em>原位成像动物模型的活组织,从而提高我们的能力,探讨在生理和病理条件下细胞行为。在这里,我们勾勒出执行小鼠腘窝淋巴结的活体成像所需的筹备工作。
Tags
Intravital Imaging
Mouse
Popliteal Lymph Node
Lymph Nodes
Immune Response
Immune Cell Interactions
Lymphocytes
T Cells
B Cells
NK Cells
Dendritic Cells
Macrophages
Bone Marrow-derived Cellular Elements
Self Antigens
Foreign Antigens
Antigen Receptors
Adhesion Molecules
Chemokines
Stromal Structures
Fibro-reticular Network
High-resolution Real-time Fluorescent In Vivo Imaging
Static Imaging
Morphology
Position
Architecture
Lymphocyte Trafficking
Dynamic Cell Behavior
Intravital Two-photon Laser Scanning
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