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Chapters
- 00:05Title
- 01:40Dissect and Prepare Tissue
- 02:36Chromatin Immunoprecipitation: Protein-DNA Conjugation
- 04:44Purification of ChIP-ed DNA
- 06:05qPCR Analysis of ChIP-DNA
- 07:25Amplify ChIP-ed DNA for High-throughput Sequencing
- 09:10Solexa Pipeline Analysis
- 10:24Endogenous Chromatin State of bam in Drosophila Testes
- 13:15Conclusions
Recently high-throughput sequencing technology has greatly increased sensitivity of Chromatin Immunoprecipitation (ChIP) experiment and prompted its application using purified cells or dissected tissue. Here we delineate a method to use ChIP technique with Drosophila tissue, which can address the endogenous chromatin state in a well-characterized biological system.
