Journal
/
/
染色质免疫沉淀(ChIP)使用果蝇组织
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
DOI:

13:47 min

March 23, 2012

, ,

Chapters

  • 00:05Title
  • 01:40Dissect and Prepare Tissue
  • 02:36Chromatin Immunoprecipitation: Protein-DNA Conjugation
  • 04:44Purification of ChIP-ed DNA
  • 06:05qPCR Analysis of ChIP-DNA
  • 07:25Amplify ChIP-ed DNA for High-throughput Sequencing
  • 09:10Solexa Pipeline Analysis
  • 10:24Endogenous Chromatin State of bam in Drosophila Testes
  • 13:15Conclusions

Summary

Automatic Translation

最近高通量测序技术,大大提高了染色质免疫沉淀(ChIP)实验的灵敏度,并促使其使用纯化的细胞或解剖组织的申请。在这里,我们划定使用芯片技术与方法<em>果蝇</em>组织,可以解决在一个特点的生物系统的内源性染色质状态。

Related Videos

Read Article