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Immunology and Infection

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艾滋病毒的成像1信封引起的病毒学突触信号合成脂双层

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers

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艾滋病毒的成像1信封引起的病毒学突触信号合成脂双层

DOI:

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11:45 min

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March 08, 2012

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Kathleen C. Prins*^1,2, Gaia Vasiliver-Shamis*^2,3, Michael Cammer², David Depoil², Michael L. Dustin, Catarina E. Hioe⁴

¹Department of Pathology,New York University Langone School of Medicine, ²Program in Molecular Pathogenesis,Marty and Helen Kimmel Center for Biology and Medicine and Skirball Institute for Biomolecular Medicine, ³Laboratory of Molecular Immunogenetics, National Institute of Arthritis and Musculoskeletal and Skin Diseases,National Institutes of Health, ⁴Veteran Affairs New York Harbor Healthcare System

Chapters

00:05Title
02:18Labeling GP120
03:52Flow Cell Assembly and Bilayer Preparation: His6-tagged GP120 DH12
06:11Injection of Cells and Immunofluorescence Staining
07:49Data Analysis
08:56Results: Imaging Lck and Fyn at HIV-1 Envelope-induced Virological Synapse
10:53Conclusion

Summary

Automatic Translation

本文介绍了一种方法，可视化的全内反射荧光显微镜（TIRF）HIV-1诱导信封上的玻璃支持平面双层病毒学突触的形成。该方法也可以结合免疫荧光染色检测艾滋病毒1信封引起的病毒学突触的形成过程中发生的信号分子的活化和再分配。

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