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共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法
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JoVE Journal Biology
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
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共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法

DOI:
10.3791/3759-v

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08:29 min

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March 27, 2012

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,
1Department of Bioengineering,University of Pittsburgh, 2Department of Chemical Engineering,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:20Culturing Human Embryonic Stem Cells
  • 02:05Definitive Endoderm and Pancreatic Progenitor Induction
  • 03:01Pancreatic Maturation
  • 03:57qRT-PCR Analysis
  • 05:06Immunocytochemistry
  • 06:21Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

目前的研究，介绍了胰腺人类胚胎干细胞分化诱导定向分化的方法。具有重要意义的发现，血管内皮细胞共培养介导派生为胰岛素表达细胞的胰腺祖细胞的人类胚胎干细胞的成熟。

Tags

Endothelial Cell Co-cultureHuman Embryonic Stem CellsDirected Differentiation ApproachPancreatic Insulin Producing CellsEmbryonic Stem CellsPluripotentCell Based TherapyRegenerative TreatmentCellular DifferentiationOrganogenesisPancreas DevelopmentEndoderm InductionNodal Pathway ModulationActivin AGrowth FactorsSonic Hedgehog InhibitionCyclopaminePancreatic MaturationNotch Signaling InhibitionVascularization

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