JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Endothelial Cell Co-culture Mediates Maturation of Human Embryonic Stem Cell to Pancreatic Insulin Producing Cells in a Directed Differentiation Approach
Please note that all translations are automatically generated.
Click here for the English version.
共培养内皮细胞介导成熟的人类胚胎干细胞向胰腺中胰岛素生成细胞在定向分化的方法
DOI:
10.3791/3759-v
•
08:29 min
•
March 27, 2012
•
Maria Jaramillo
,
Ipsita Banerjee
2
1
Department of Bioengineering
,
University of Pittsburgh
,
2
Department of Chemical Engineering
,
University of Pittsburgh
Chapters
00:05
Title
01:20
Culturing Human Embryonic Stem Cells
02:05
Definitive Endoderm and Pancreatic Progenitor Induction
03:01
Pancreatic Maturation
03:57
qRT-PCR Analysis
05:06
Immunocytochemistry
06:21
Analysis of Differentiation of Human Embryonic Stem Cells into Insulin-producing Cells
08:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
目前的研究,介绍了胰腺人类胚胎干细胞分化诱导定向分化的方法。具有重要意义的发现,血管内皮细胞共培养介导派生为胰岛素表达细胞的胰腺祖细胞的人类胚胎干细胞的成熟。
Tags
Endothelial Cell Co-culture
Human Embryonic Stem Cells
Directed Differentiation Approach
Pancreatic Insulin Producing Cells
Embryonic Stem Cells
Pluripotent
Cell Based Therapy
Regenerative Treatment
Cellular Differentiation
Organogenesis
Pancreas Development
Endoderm Induction
Nodal Pathway Modulation
Activin A
Growth Factors
Sonic Hedgehog Inhibition
Cyclopamine
Pancreatic Maturation
Notch Signaling Inhibition
Vascularization
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/3759?article_type=v
从小鼠胚胎干细胞的造血干细胞的推导
check_url/3759?article_type=v
人类胚胎干细胞的推导Immunosurgery
check_url/3759?article_type=v
胚胎干细胞衍生的内皮细胞治疗肢体缺血
check_url/3759?article_type=v
成人内皮细胞集落形成祖细胞的分离和大型规模扩张
check_url/3759?article_type=v
隔离和动物血清自由膨胀的人脐血源性间质基质细胞(MSCs),内皮细胞集落形成祖细胞(ECFCs)
check_url/3759?article_type=v
视频人类胚胎干细胞克隆生长的生物信息学分析
check_url/3759?article_type=v
胚胎干细胞分化成少突胶质前体
check_url/3759?article_type=v
人类胚胎干细胞畸胎瘤形成命运的映射
check_url/3759?article_type=v
高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞
check_url/3759?article_type=v
人类体细胞重编程为诱导多能干细胞(iPS细胞)用绿色荧光蛋白逆转录病毒载体
Read Article