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利用同位素标记的质谱剖析巯基氧化还原蛋白质

JoVE Journal
Biology

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JoVE Journal Biology

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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利用同位素标记的质谱剖析巯基氧化还原蛋白质

DOI:

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12:07 min

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March 24, 2012

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Jennifer Parker, Ning Zhu, Mengmeng Zhu, Sixue Chen^2,3,4

¹Plant Molecular and Cellular Biology Program,University of Florida , ²Department of Biology,University of Florida , ³Interdisciplinary Center for Biotechnology Research,University of Florida , ⁴Genetics Institute,University of Florida

Chapters

00:05Title
02:07Inoculation of Tomato
03:13Protein Extraction
04:21Sample Preparation and Peptide Labeling with cysTMT
06:07Removal of Non-reacted Tag Sample Fractionation
07:44Sample Enrichment of cysTMT-labeled Peptides
08:43Mass Spectrometry Analysis
10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
11:11Conclusion

Summary

Automatic Translation

活性氧水平升高，当细胞遇到压力条件。在这里，我们表明3'-3'二氨基联苯胺染色的例子，以及cysTMT标签和质谱分析的氧化还原蛋白质在<em>野火</em>处理番茄叶片。

Tags

Thiol Redox Proteome Isotope Tagging Mass Spectrometry Pseudomonas Syringae Pv. Tomato Bacterial Speck Disease Solanum Lycopersicum Brassica Species Arabidopsis Thaliana Reactive Oxygen Species (ROS)Hydrogen Peroxide DAB Staining Redox Status Of Proteins Cysteine Oxidation Tandem Mass Tag (TMT) Reagents Cysteine-reactive TMT (cysTMT) Tags

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