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衍生Aldononitrile乙酸氨基葡萄糖和胞壁酸的微生物在土壤中残留量的测定气相色谱的检测
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JoVE Journal
Biology
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Please note that all translations are automatically generated.
Click here for the English version.
衍生Aldononitrile乙酸氨基葡萄糖和胞壁酸的微生物在土壤中残留量的测定气相色谱的检测
DOI:
10.3791/3767-v
•
11:06 min
•
May 19, 2012
•
Chao Liang
2
,
Harry W. Read
,
Teri C. Balser
3
1
DOE-Great Lakes Bioenergy Research Center
,
University of Wisconsin, Madison
,
2
Department of Soil Science
,
University of Wisconsin, Madison
,
3
Department of Soil and Water Science
,
University of Florida
Chapters
00:05
Title
01:52
Sample Preparation and Purification
05:06
Derivatization
06:06
Separation and Measurement
08:11
Representative Results
09:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个协议aldonitrile从土壤中提取的葡萄糖和胞壁酸醋酸衍生物GC为基础的分析方法。对于澄清的化学机制,我们也提出一项战略,以确认衍生工具及电子电离后形成的碎片离子的结构。
Tags
GC-based Detection
Aldononitrile Acetate Derivatized
Glucosamine
Muramic Acid
Microbial Residue Determination
Soil Microbial Analysis
Biomarker Analysis
Glucosamine And Muramic Acid Analysis
Analytical Methods
Derivatization
GC-based Analysis
Precision
Sensitivity
Chromatographic Separation
Sample Storage
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