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人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体

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JoVE Journal Biology

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

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人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体

DOI:

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08:25 min

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April 03, 2012

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Kun-Yong Kim, Eriona Hysolli, In-Hyun Park

¹Yale Stem Cell Center, Department of Genetics,Yale School of Medicine

Chapters

00:05Title
01:28Infecting Cells with Retrovirus Expression Reprogramming Factors
03:31Isolation and Expansion of Induced Pluripotent Stem Cells
04:42Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
06:06Representative Cell-reprogramming Results
08:03Conclusion

Summary

Automatic Translation

的方法来产生人类诱导多能干细胞（iPS细胞）通过逆转录病毒介导的异位表达Oct4的，SOX2的，KLF4和MYC的描述。基于GFP的表达，以确定人类IPSC的殖民地一个切实可行的办法进行了讨论。

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