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Omprogrammering humane somatiske celler i induceret pluripotente stamceller (IPSC'er) under anvendelse af retroviral vektor med GFP
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JoVE Journal Biology
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Omprogrammering humane somatiske celler i induceret pluripotente stamceller (IPSC'er) under anvendelse af retroviral vektor med GFP

DOI:
10.3791/3804-v

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08:25 min

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April 03, 2012

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, ,
1Yale Stem Cell Center, Department of Genetics,Yale School of Medicine

Chapters

  • 00:05Title
  • 01:28Infecting Cells with Retrovirus Expression Reprogramming Factors
  • 03:31Isolation and Expansion of Induced Pluripotent Stem Cells
  • 04:42Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
  • 06:06Representative Cell-reprogramming Results
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

En fremgangsmåde til at generere humane inducerede pluripotente stamceller (IPSC'er) via retrovirus-medieret ektopisk ekspression af OCT4 er SOX2, KLF4 og MYC beskrevet. En praktisk måde at identificere menneskelige IPSC kolonier baseret på GFP-ekspression er også diskuteret.

Tags

ReprogrammingHuman Somatic CellsInduced Pluripotent Stem CellsRetroviral VectorGFPHuman Embryonic Stem CellsPluripotentIn Vitro Disease ModelingRegenerative MedicineTranscription FactorsOct4Sox2Klf4MycAutologous CellsFibroblast CellsGFP-containing Retroviral BackboneESC Culture ConditionMorphologyRetroviral Transgene SilencingFluorescence MicroscopePluripotency GenesSurface Markers

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